pcaggs backbone Search Results


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New England Biolabs pcag ires gfp backbone
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Addgene inc pcagig backbone
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Addgene inc vector backbone pcag spcas9 gfp u6 grna
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Addgene inc pcag vector backbone
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Addgene inc plasmid 92141 backbone
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Addgene inc pcag archon1 kgc egfp er2 wpre
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Image Search Results


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Journal: Molecular cell

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit

doi: 10.1016/j.molcel.2020.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fully Sequenced cDNA (Source BioScience) with primers forward 5′-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3′ and reverse 5′-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3′, followed by cloning into the pCAG-IRES-GFP backbone ( Ambrozkiewicz et al., 2018 ) with the restriction enzyme EcoRI (NEB).

Techniques: Recombinant, Luciferase, shRNA, Transfection, Protease Inhibitor, Plasmid Preparation, Mass Spectrometry, RNA Sequencing Assay, Software, Over Expression

KEY RESOURCES TABLE

Journal: Molecular cell

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit

doi: 10.1016/j.molcel.2020.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fully Sequenced cDNA (Source BioScience) with primers forward 5′-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3′ and reverse 5′-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3′, followed by cloning into the pCAG-IRES-GFP backbone ( Ambrozkiewicz et al., 2018 ) with the restriction enzyme EcoRI (NEB).

Techniques: Recombinant, Luciferase, shRNA, Transfection, Protease Inhibitor, Plasmid Preparation, Mass Spectrometry, RNA Sequencing Assay, Software, Over Expression